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Breed Material (WP2.2)

Breed Material
Technological developments to improve the cryopreservation of reproductive/breed materials

Developments are shared between nodes having reproductive collections (Partners 1, 2, 3, 5).

Cells maintained in the National Cryobank must keep a high level of reproductive efficiency in order to reintroduce and stabilize donor genomes in host populations. An important issue is also to speed up the reconstitution of an entire genome which questions the regeneration technology to be used.

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Maintenance of the reproductive potential of stored cells is the first critical point. One second key point is that genetic, epigenetic and phenotypic conformity of the donor animal must be recovered in the offspring obtained from the cryopreserved cells. Finally, optimal biosecurity of the methods developed to store the cells is the third key point of success of cryobanking to ensure safety conditions while re-introducing.

The aims of the work package are to 1) improve and standardize the handling and freezing-thawing procedures for cryobanking of different cell types, 2) better assess their reproductive potential after thawing and explore the related regeneration methods (fertilization, transplantation, reprogramming including induced pluripotent stem cells) when these are not efficient enough and 3) assess the epigenome integrity. All the processes developed to secure the biotechnology of cell storage will include tasks on the substitution of protective molecules from animal origin by chemically defined/synthetic medium (Pillet et al., 2011) and on optimized conditions to prevent contamination during storage.

All experimental protocols will be submitted for approval to ethical committees, according to regulations. In addition, possible societal issues will be discussed within the Stakeholders committee and the scientific committee of CRB-Anim before developing new procedures on embryos and somatic cells. Developments will be undertaken by INRA partners and VetAgroSup in collaboration with IFREMER, SYSAAF and INSERM (AgrobioStem) depending on the species and the cell type.

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For some species, the cryopreservation procedures are not efficient enough for cryobanking of sperm and offspring production.
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Diploïd germ cells including whole gonadic tissues

Cryopreservation procedures
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Embryos & Larvae

Cryopreservation of invertebrates larvae and embryo in honey bee, great scallop (Pecten maximus), and pacific oyster (Crassostrea gigas) will be...
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Somatic Cells & Tissues

Coming Soon
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